Table 7. Effect of normalization strategy on statistical parameters calculated for Nppb and Vcam1 expression using combinations of multiple reference genes.

		NF3	NF4	NF5	NF6	NF7	NF8	NF9	NF10
Nppb	p-value	0.003	0.009	0.007	0.009	0.035	0.029	0.043	0.075
	power	0.954	0.926	0.848	0.748	0.7	0.652	0.417	0.252
	n	7	8	9	12	13	14	24	43
Vcam1	p-value	<0.001	0.001	0.001	0.001	0.003	0.007	0.007	0.002
	power	0.999	0.991	0.993	0.962	0.891	0.835	0.857	0.953
	n	5	6	6	7	9	10	9	7

Two-tailed Mann-Whitney U test was used for calculating p-values. The statistical power was calculated for a Student's t-test using statistical parameters of log₂ transformed expression data. Sample size (n) is number per group needed to obtain a power of at least 0.8. Nppb: group 2 vs. group 3; Vcam1: group 1 vs. group 3.