A. Shh and Sox9 transcripts levels are shown for E16.5 testes and adult male adrenals, respectively. Statistical significance was determined by one-way ANOVA. B. Comparison of adult adrenals obtained from different allelic combinations as indicated, by either tyrosine hydroxylase staining of adrenal medulla (green), or AKR1C18/20α-HSD staining, which marks cells of the fetal X-zone (brown, black arrows). The eccentric smaller medulla observed in heterozygous Sf-1+/− adrenal is further exaggerated in Sf-12KR/− adrenals.