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The effects of Nox2 siRNA or Nox2 KO on alveolar cell O2•− production and cell-cycle progression. (A) Wild-type alveolar epithelial cells. (B) Nox2 KO alveolar epithelial cells. NADPH-dependent O2•− production was measured by lucigenin chemiluminescence. Cell cycle progression was measured by flow cytometry. *P < 0.05 for indicated values versus control vehicle values; n = 3 independent cell isolations.
