Figure 1.
In vitro binding of NF-κB DNA decoys to the NF-κB protein complex. Nuclear extracts were isolated from NF-κB/SEAP HEK 293 cells and incubated with radiolabelled NF-κB DNA decoys at room temperature for 20 min. Samples were analyzed on a 16% native PAGE gel and imaged with a Typhoon 7000 phosphorimager.