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. Author manuscript; available in PMC: 2012 Aug 24.
Published in final edited form as: J Am Chem Soc. 2011 Jul 29;133(33):13176–13182. doi: 10.1021/ja204980v

Figure 2.

Figure 2

Light-activation of NF-κB DNA decoy binding. Nuclear extracts were isolated from NF-κB/SEAP HEK 293 cells and caged decoys were irradiated for 1 min (365 nm, 25 W) and incubated with nuclear extracts at room temperature for 20 min. Samples were analyzed on a 16 % Native PAGE gel and imaged with a Typhoon 7000 phosphorimager.