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. Author manuscript; available in PMC: 2012 Aug 24.
Published in final edited form as: J Am Chem Soc. 2011 Jul 29;133(33):13176–13182. doi: 10.1021/ja204980v

Figure 4.

Figure 4

Temporally controlled deactivation of SEAP expression with caged NF-κB decoys. NF-κB/SEAPorter HEK293 cells were transfected with inactive (D0), non-caged (D5), and caged (D6) DNA decoys using X-tremeGENE, followed by TNFα addition 4 hrs post-transfection. Aliquots of the supernatant were taken at 1, 8, 12, 24 and 48 hrs after induction with TNFα followed by SEAP quantification. Cells were irradiated for 2 min (365 nm, 25 W) at 12 hrs after induction. The SEAP signal (Phospha Light Systems kit, Applied Biosystems) was normalized to cell viability (Cell Titer Glo, Promega). All experiments were performed in triplicate and error bars represent standard deviations. Asterisks indicate t-test results: * p < 0.5, ** p < 0.05, and *** p < 0.0005.