Table 1.
DNA | Sequence | Tm/°C | |
---|---|---|---|
−UV | +UV | ||
D0 | 5′ T* T* G* C* C* G* T* A* C* C* T* G* A* C* T A* A* C* G* G* C* A* T* G* G* A* C* T* G* A |
52.1 ± 0.5 | - |
| |||
D1 | 5′ T* T* G* G* G* G* A* C* T* T* T* C* C* A* G A* A* C* C* C* C* T* G* A* A* A* G* G* T* C |
45.8 ± 1.5 | - |
| |||
D2 | 84.4 ± 0.0 | - | |
| |||
D3 | 54.4 ± 1.7 | 81.8±0.6 | |
| |||
D4 | NA | 83.1 ± 0.6 | |
| |||
D5 | 74.8 ± 0.6 | - | |
| |||
D6 | NA | 73.8 ± 1.5 | |
| |||
D7 | NA | 74.4 ± 0.9 |
Indicates a phosphorothioate bond; a bold T denotes a caged thymidine nucleotide; NA indicates that no melting temperature could be measured and thus no formation of dsDNA was observed.