Supplementary Fig. 2.

Spib morpholino comparison of bilateral versus dorsal blastomere injection. (A–F): Tailbud stage 23 embryos injected with Xtspib-e1i1MO, 40 ng dose, into both blastomeres at the two-cell stage (2/2, A and B), into dorsal blastomeres (C and D), or non-injected control (E and F). Embryos hybridized with probes for mpo and nkx2-5. (G–L): Late tailbud stage 28 embryos, with the same sequence of spib morpholino blastomere injections presented and hybridized to mpo and tbx5. (M–R): Stage 31 tadpoles with the same sequence of spib morpholino blastomere injections and hybridized to mpo and tnni3. Different morpholino injected embryos were chosen for presentation compared to Fig. 2 and Fig. 3.