Supplementary Fig. 7.

Lurp1 mismatch morpholino does not affect Xenopus development. (A–D) Stage 24 embryos injected with the mismatch control morpholino, lurp1-misMO, into both blastomeres at the two-cell stage (A and B), 12 ng dose, or into dorsal blastomeres (C and D). Embryos hybridized to mpo and nkx2-5. (E and F) Sibling embryo injected with the active lurp1 morpholino into dorsal blastomeres at 12 ng dose. (G–J) Stage 31 tadpoles with the same sequence of mismatch control lurp1 morpholino and hybridized to mpo and mlc2. (K and L) Sibling tadpole injected with the active lurp1 morpholino. Myeloid cell production and embryo development is unaffected by the mismatch control morpholino whereas the active morpholino causes macrophage migration from lateral positions and defective (broader) heart myocardium morphogenesis.