Figure 3. Reduced expression of FILIP1L mRNA in invasive serous carcinomas compared with non-invasive serous borderline tumors.
Real-time RT-PCR analysis for FILIP1L on cDNA from ovarian serous carcinomas and ovarian serous borderline tumors. The y axis represents FILIP1L mRNA expression which was standardized with the housekeeping gene hRPL7. Error bars indicate SEM (n = 10 for serous borderline tumors and n = 17 for serous carcinoma). P value comparison was: p<0.0001 for TaqMan probe A (A); p<0.0001 for TaqMan probe B (B). C, Immunohistochemical staining of FILIP1L in ovarian serous carcinomas and ovarian serous borderline tumors is shown. Scale bar shown indicates 0.1 mm. The result is a representative image from ten independent tissue specimens each. D, FILIP1L expression in invasive serous carcinomas was significantly less than that in non-invasive serous borderline tumors (p = 0.0044). Ten specimens from each group were immunohistochemically stained and the staining was scored in a blinded fashion. Error bars indicate SEM. Expression score was performed as described in Materials and Methods.