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. Author manuscript; available in PMC: 2012 Mar 21.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Sep 21;3(2):162–173. doi: 10.1002/wnan.116

FIGURE 6.

FIGURE 6

Preparation and characterization of manganese nanobialys: (Top left) TEM (drop deposited over nickel grid, 1% uranyl acetate) and (top right) atomic force microscope (AFM) image of nanobialys (drop deposited over glass) Reaction conditions: (1) anhydrous chloroform, gentle vortexing, room temperature; (2) aqueous solution of 2 [Mn(III)-protoporphyrin], inversion, room temperature, filter using short bed of sodium sulfate and cotton; (3) Biotin-Caproyl-PE, filter mixed organic solution using cotton bed, 0.2 µM water, vortex, gently evaporation of chloroform at 45°C, 420 mbar, 0.2 µM water, sonic bath, 50°C, 1/2 h, dialysis (2 kDa MWCO cellulose membrane) against water. (Bottom) MRI images of fibrin-targeted nanobialys (right) or control nanoparticles (bottom left) and (bottom middle) bound to cylindrical plasma clots measured at 3 T.