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Amplification plots of JAK2V617F real-time PCR of 25 (A) and 22 (B) healthy individuals in duplicate (JAK2V617F negative cohort) and one 1% JAK2V617F positive control. The amplification plots shown are representative of the entire cohort. Gray horizontal line (at Delta Rn 0.8e-002) indicates threshold; black vertical line, cutoff CT value of 37.9.
