Table 2.

Sequences of Primers, Detection Probes, and PNA and LNA Blocking Oligonucleotides to Detect and Quantify JAK2V617F and to Detect Albumin by Real-Time PCR

JAK2 V617F: primers and mutant probe41	Sequences
Forward primer	5′-AAGCTTTCTCACAAGCATTTGGTTT-3′
Reverse primer	5′-AGAAAGGCATTAGAAAGCCTGTAGTT-3′
Mutant probe	5′-FAM-TCCACAGAAACATAC-MGB-BHQ-3′
LNA wild-type blocking probe	5′-ACAGACACATA-3′
PNA wild-type blocking probe	5′-Acetyl-CTCCACAGACACATAC-3′

Albumin: primers and probe [53]	Sequences
Forward primer	5′-TGAAACATACGTTCCCAAAGAGTTT-3′
Reverse primer	5′-CTCTCCTTCTCAGAAAGTGTGCATAT-3′
Probe	5′-FAM-TGCTGAAACATTCACCTTCCATGCAGA-TAMRA-3′

BHQ, black hole quencher; MGB, minor groove binder.