Table 1.
Method (n = 195) |
||||
---|---|---|---|---|
Variable | TaqMan | dHPLC | HRM | Direct sequencing |
Suitable for evaluation [no. (%)]⁎ | 189 (96.9) | 182 (93.3) | 173 (88.7) | 172 (88.2) |
V600 WT | 167 | 160 | 152† | 146 |
V600E [no. (%)]‡ | 22 (11.6) | 18 (9.9) | 20 (11.6) | 17 (9.9) |
Mutation | ||||
c.1800G>A | NAD | NAD | NAD | 1 |
G581D | NAD | 1 | NAD | 1 |
G596D | NAD | 1 | NAD | 1 |
G596S | NAD | 1 | NAD | 3 |
A598T+W604X | NAD | 1 | NAD | 1 |
S602F | NAD | NAD | NAD | 1 |
R603X | NAD | NAD | 1 | 1 |
H608Y§ | NAD | NAD | NAD | 1 |
Unsuitable for evaluation | 6 | 13 | 22 | 23 |
NAD, not able to be detected by this technique.
Referred to the total number of cases (n=195).
Including four non-V600E assigned mutations.
Referred to the number of cases suitable for evaluation.
H608Y was found in a specimen carrying a V600E mutation.