Table 1.
BRAF Mutations Found Using TaqMan, dHPLC, HRM, and Direct Sequencing
| Method (n = 195) |
||||
|---|---|---|---|---|
| Variable | TaqMan | dHPLC | HRM | Direct sequencing |
| Suitable for evaluation [no. (%)]⁎ | 189 (96.9) | 182 (93.3) | 173 (88.7) | 172 (88.2) |
| V600 WT | 167 | 160 | 152† | 146 |
| V600E [no. (%)]‡ | 22 (11.6) | 18 (9.9) | 20 (11.6) | 17 (9.9) |
| Mutation | ||||
| c.1800G>A | NAD | NAD | NAD | 1 |
| G581D | NAD | 1 | NAD | 1 |
| G596D | NAD | 1 | NAD | 1 |
| G596S | NAD | 1 | NAD | 3 |
| A598T+W604X | NAD | 1 | NAD | 1 |
| S602F | NAD | NAD | NAD | 1 |
| R603X | NAD | NAD | 1 | 1 |
| H608Y§ | NAD | NAD | NAD | 1 |
| Unsuitable for evaluation | 6 | 13 | 22 | 23 |
NAD, not able to be detected by this technique.
⁎
Referred to the total number of cases (n=195).
†
Including four non-V600E assigned mutations.
‡
Referred to the number of cases suitable for evaluation.
§
H608Y was found in a specimen carrying a V600E mutation.