Table 2.
Concordance and Performance Indexes between dHPLC and HRM versus TaqMan and Direct Sequencing in V600E Detection
| dHPLC |
HRM |
|||||||
|---|---|---|---|---|---|---|---|---|
| Method | Positive (no.) | Negative (no.) | Sensitivity [% (95% CI)] | Specificity [% (95% CI)] | Positive (no.) | Negative (no.) | Sensitivity [% (95% CI)] | Specificity [% (95% CI)] |
| TaqMan | ||||||||
| Positive | 13 | 2 | 90 (77.4–100) | 100 (99.7–100) | 16 | 3 | 84.2 (65.2–100) | 97.4 (94.5–100) |
| Negative | 0 | 151 | 4 | 147 | ||||
| Direct sequencing | ||||||||
| Positive | 12 | 1 | 93.8 (78.8–100) | 98.6 (96.4–100) | 14 | 2 | 87.5 (68.2–100) | 95.7 (91.9–99.4) |
| Negative | 1 | 136 | 6 | 132 | ||||