Table 2.
Oligonucleotide Sequences Used for Amplicons, Primers, Pleiades Probes, and IS
| Gene | Amplicon sequence |
|---|---|
| ANXA5 | 5′-CGAGACTTCTGGCAATTTAGAGCAACTACTCCTTGCTGTTGTGAAATCTATT(A)CGAAGT(A)ATACCTGCCTACCTTGCAGAGACC-3′ |
| CASC3 | 5′-TCCTGCAACCACGGGAACTTCGAGGTATGCCCAACCATATACACAT(A)GGGAGCA(T)GGACCTCCACCTCAGTTTAACCGG-3′ |
| CPEB4 | 5′-ACAGCCACTTGACCCACGAAAAACTATATTTGTTGGTGGTGTTCCTCGACCAT(A)TAC(G)GAGCTGTGGAGCTTGCGATGATAATGG-3′ |
| DUSP6 | 5′-GCTGCTGCTGATGGACTGCCGGCCGC(G)AGGAG(C)CTATACGAGTCGTCGCACATCGAGTCGGCCATCAACGTGG-3′ |
| ERBB3 | 5′-TGCTCACGGGACACAATGCCGACCTCTCCTTCCTGCAGT(C)GGATTCG(A)AGAAGTGACAGGCTATGTCCTCGTG-3′ |
| HMMR | 5′-TAAGGCGCCCTTGAAACGATTCAATGACCCTTCTGGTT(A)GTG(C)CACCATCTCCAGGTGCTTATGATGT-3′ |
| IRF4 | 5′-CAGGACTACAACCGCGAGGAGGACGCCGCGCTCTTCAAGGCTTGGGCACTGTTTAAAGGA(T)AAGTTCCGAGAAGGCATCGACA-3′ |
| MMD | 5′-ATGGCCGCTACAAGCCAACTTGCTATGAACATGCTGCTAACTGTTACACACACG(A)CAT(C)TCCTCATTGTTCCGGCCAT-3′ |
| NF1 | 5′-GGCCAATGTGGTTCCTTGTTCTCAGTGGGATGAACTAGCTCGAGTTCT(A)GGTT(A)ACTCTGTTTGATTCTCGGCATTTACTC-3′ |
| PPIA | 5′-TCCATGGCAAATGCTGGACCCAACACAAATGGTTCCCAGTTTTTCATCT(A)GCACTGCC(G)AAGACTGAGTGGTTGGATGGCAAGC-3′ |
| STAT2 | 5′-TCATACTAGGGACGGGAAGTCGCGACCAGAGCCATTGGAGGGCGCGGGGACTGCAACCCT(A)AAT(A)CAGCAGAGCCCAAATGGCGCAG-3′ |
| TBP | 5′-GCCCGAAACGCCGAATATAATCCCAAGCGGTTTGCTGCGGTAATCAT(A)GAGGAT(A)AAGAGAGCCACGAACCACGGCACTGATT-3′ |
| UBE2D2 | 5′-TGCCTGAGATTGCTCGGATCTACAAAACAGATAGAGAAAAGTACAACAGAAT(A)AGCT(A)CGGGAATGGACTCAGAAGTATGCGA-3′ |
| ZNF264 | 5′-TGTGGGCTCCTGGTGTCTCTGGGGTGTCCTGTTCCCAAAGCTGAGCTGA(T)TCTGC(G)CACCTAGAGCATGGGCAGGA-3′ |
| ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | |
| DLG2 | 5′-CGCAACTCGTCAGCCTTCAA(T)TGA(T)CTCTCCAACGGGCCGTCTCCCTGGAAGGAGAGCCTCGCAAGGTAGTCCTGCACAAAGGC-3′ |
| GUSB | 5′-CTCATTTGGAATTTTGCCGATTTCA(T)TGA(T)CTGAACAGTCACCGACGAGAGTGCTGGGGAATAAAAAGGGGATCTTCACTCGG-3′ |
| HGF | 5′-AAGTCTGTGACATTCCTCAGTGTTCAGAAGTTGA(G)A(G)TGCATGACCTGCAATGGGGAGAGTTATCGAGGTCTCATGGA-3′ |
| LCK | 5′-GAGCCCATCTACATCATCACTGAATACA(T)TGGA(T)GAATGGGAGTCTAGTGGATTTTCTCAAGACCCCTTCAGGCA-3′ |
| MTOR | 5′-AACAAGCGATCCCGAACGAGGA(T)CGGA(T)TTCCTACTCTGCTGGCCAGTCAGTCGAAATTTTGGACGGTGTGGAACTTGG-3′ |
| RNF4 | 5′-GCCTGTGGTGGTTGATCTGACTCAC(T)AATGA(G)CTCTGTTGTGATTGTTGACGAAAGAAGAAGACCAAGGAGGAATGCTAGGAGGCTG-3′ |
| STAT1 | 5′-GGGAAGGGGCCATCACATTCACATG(C)GGTGGA(T)GCGGTCCCAGAACGGAGGCGAACCTGACTTCCATGCGGTTG-3′ |
Single underline, forward primers; double underline, reverse primers; bold, pleiades probes; parentheses, nucleotide change in IS sequence to the left.
Above dashed line: forward primer correlates to limited primer; below dashed line: reverse primer correlates to limited primer.