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. 2011 Sep;13(5):474–484. doi: 10.1016/j.jmoldx.2011.05.005

Figure 1.

Figure 1

Detection of p53 codon 249 mutations by an LNA clamp-mediated PCR assay and melting curve analysis with SimpleProbes. A: Locations and sequences of primers (arrows), clamp (underlined), and probes (shaded) used in the assay. Codons 248 and 249 are indicated by vertical lines. LNAs are italicized. SimpleProbe sequences shown with LNA are italicized and capitalized. PCR products derived from plasmids p53 WT, p53_249T, p53_249C, or H2O (arrows) were determined by melting curve analysis with the SimpleProbes 249WT (B), 249T (C), and 249C (D).