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. 2011 Sep;13(5):474–484. doi: 10.1016/j.jmoldx.2011.05.005

Figure 2.

Figure 2

Selective suppression of PCR amplification of TP53 WT templates by the LNA clamp. Melting curve analysis of the PCR products generated from the p53_WT (gray) or p53_249T (black) template in the absence (-) or presence (+) of the LNA clamp using SimpleProbe SP_249T. A and B: The PCR products were generated from a range of 101 to 107 copies of WT (gray) templates, with 10 copies of the p53_249T (black) template in each reaction. The ratios of p53_249T/p53_WT are indicated. C and D: Melting curve analysis of the PCR products generated from a range of 101 to 107 copies of the mutated p53_249T template with 107 copies of the p53_WT template. The ratios of p53_249T/p53_WT are indicated.