Table 2.
Primer and probe name | Nucleotide location | Sequence |
---|---|---|
P53_qF | 14050–14065 | 5′-CTGCATGGGCGGCATG-3′ |
P53_249R | 14076–14090 | 5′-TGATGGTGAGGATGG-3′ |
P53_LNA | 14070–14076 | 5′-GGAGGCC-3′ |
P53_249_T probe | 14067–14078 | 5′-ACCGGAGTCCCA-3′ |
P53_249_C probe | 14067–14078 | 5′-ACCGGAGCCCCA-3′ |
P53_249_WT probe | 14067–14078 | 5′-ACCGGAGGCCCA-3′ |
TP53 is GenBank no. X54156. The boldfaced and italicized bases denote LNA bases.