Table 3.

Detection of p53 Mutations in HCC Tissues by the TP53 249 Assay

Tissue	Tm by SP_249T		Sequence (5′-CCGGAGGCCCA-3′)
	Without LNA	With LNA
1	48°C (WT)	–	ND
2	48°C (WT)	58°C	5′-CCGGAGCCCCA-3′
3	48°C (WT)	63°C	5′-CCGGAGTCCCA-3′
4	48°C (WT)	–⁎	ND
5	48°C (WT)	–	ND
6	48°C (WT)	–	ND
7	48°C (WT)	–	ND
8	48°C (WT)	–	ND
9	48°C /57°C	63°C	5′-CCGGAGTCCCA-3′
10	48°C (WT)	63°C	ND
11	48°C (WT)	60°C	5′-CCGGAGTCCCA-3′
12	48°C (WT)		Insertion†
13	48°C (WT)	63°C	5′-CCGAAGGCCCA-3′
14	48°C (WT)	–	ND
15	48°C (WT)	–	ND

The boldfaced and underlined mutations are those detected by DNA sequencing, and the italicized and underlined sequence is that of the LNA clamp.

ND, not determined.

^⁎No peak detected.

^†Identified as underlined in the following sequences: 5′-CATGA-ACCGGAGGC-CCA-3′ and 5′-CATGA-TGTTCCCAGCAGGCTACACGCTCCTT-CCA-3′.