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. Author manuscript; available in PMC: 2011 Aug 18.
Published in final edited form as: Cell Mol Bioeng. 2011 Jun 1;2(4):138–147. doi: 10.1007/s12195-011-0161-3

FIGURE 2.

FIGURE 2

Targeting of FRET biosensors into microdomains of plasma membrane. The targeting signal into lipid rafts (Lyn-tag) contains the acylation substrate derived from Lyn kinase. N-terminal glycine and cysteine can be myristoylated and palmitoylated. The tagged protein modified with these long saturated fatty acids can be incorporated into the lipid-raft microdomains of the plasma membrane. Non-raft targeting signal (KRas-tag) was adopted from KRas which has polybasic amino acids and a C-terminal cysteine site for prenylation modification. The tagged protein with this long unsaturated fatty acids and positively charged amino acids can be attracted to the general membrane regions of the plasma membrane, but not into lipid rafts.