Figure 1.

Setup and representative data for ultrasound evaluation of brachial FMD. (a) A sterotatic clamp with micrometer adjustment is used to hold the ultrasound probe. The occluding cuff is positioned just below the elbow. (b) Doppler trace showing flow velocity profile during early reactive hyperemia. (c) B-mode image of brachial artery demonstrating selection of region of interest and edge-detection analysis with Brachial analyzer software package. (d) Brachial artery diameter profile during FMD protocol as measured using brachial analyzer the software package (0-1 minute baseline, 1–6 minutes cuff up, 6–11 minutes response to reactive hyperemia (FMD)).