Figure 10.

Suppression of pVHL prevents TGF-β1-mediated fibroblast proliferation. A) vhlh 2-lox MEFs were infected with pAd-Null or pAd-Cre at 50 pfu/cell for 1 d, and amounts of pVHL and Cre were determined by Western blot analysis. Actin was used as loading control. B) After adenoviral infection, vhlh 2-lox MEFs were treated with TGF-β1 (2 or 5 ng/ml) for 8 h and labeled with BrdU for another 16 h. Rate of BrdU incorporation was normalized to the control group. Data are expressed as means + se. n = 3. *P < 0.05; **P < 0.01.