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. 2011 Sep;25(9):3032–3044. doi: 10.1096/fj.10-177824

Figure 2.

Figure 2.

Lungs from bleomycin-treated mice express elevated pVHL levels. A) Paraffin-embedded lung samples from PBS- and bleomycin-treated mice were subjected to trichrome staining. Arrows indicate collagen staining. B) At 21 d after administration of PBS or bleomycin, mouse lungs were collected and used for RNA extraction. Mouse pVHL mRNA levels were determined by qRT-PCR. RPL19 gene was used as internal control for qRT-PCR. pVHL/RPL19 ratio of PBS-treated mice (n=3) was set to 100, and pVHL/RPL19 ratio of bleomycin-treated mice (n=4) was normalized to that of PBS-treated mice. C, D) At 14 d after administration with PBS or bleomycin, mouse lungs were used to culture mouse lung fibroblasts (C) and ATII cells (D). Mouse pVHL mRNA levels were determined by qRT-PCR. RPL19 gene was used as internal control for qRT-PCR. pVHL/RPL19 ratio of PBS-treated mice (C, n=4; D, n=9) was set to 100, and pVHL/RPL19 ratio of bleomycin-treated mice (C, n=4; D, n=9) was normalized to that of PBS-treated mice. Graphs represent means + se. *P < 0.05; **P < 0.01.