Figure 4.

Overexpression of pVHL increases integrin α5 expression in N12 primary human lung fibroblasts. A) N12 human primary lung fibroblasts were infected with pAd-Null or pAd-VHL-HA for 2 d, and mRNA expression levels of integrin α5, αv, α4, and β1 were determined by qRT-PCR; RPL19 gene was used as internal control. B–F) Protein expression levels of integrin α5 (B), α4 (C), β1 (D), α1 (E), and α2 (F) were determined by Western blot analysis. Tubulin was used as control for equal loading. Overexpression of exogenous pVHL-HA was confirmed by Western blot analysis. Measurement of uninfected (A) or pAd-Null-infected group (B–F) was set to 100; graphs represent means + se of ≥3 independent experiments. n = 4 (A, C, D); 6 (B); 3 (E–F). *P < 0.05.