Table 2.
Sequence information of PCR gene targets
Name | Gene | Chr | Sequence |
---|---|---|---|
Displacement loop | MT-Dloop | mt | F: AGCACATTACAGTCAAATCCCTTCTC |
R: CACGGAGGATGGTGGTCAAG | |||
P: CCCCATGGATGACCCC | |||
β-2-Microglobulin | B2M | 15 | F: GTGCCTGATATAGCTTGACACCAA |
R: TCGGGAAAAGACACATTAATATTGCCA | |||
P: CCCCAAGTGAAATACC | |||
Mitochondrial encoded 16S RNA | MT-RNR2 | mt | Proprietary sequence assay Hs02596860_s1 |
TATA box binding protein | TAF-1L | X | Proprietary sequence assay Hs00542346_s1 |
Primers and probes for MT-Dloop and B2M were custom designed and are reported. MT-RNR2 and TAF-1L were purchased as proprietary sequences from Applied Biosystems. F, forward; R, reverse; P, probe.