Table 1.
Irradiation conditions for mouse genomic DNA at a series of UV wavelengths
| Sample | Actual λ (nm)a | FWHM (nm) | Spot size (cm2) | Power (mW) | Irradiation time (s) | Dose (J/cm2)b | Attenuation correctionc |
|---|---|---|---|---|---|---|---|
| 1 | 349 | 4.1 | 0.319 | 0.50 | 319 | 0.5 | 1.00 |
| 3 | 339 | 3.6 | 0.245 | 0.38 | 323 | 0.5 | 1.00 |
| 2 | 330 | 2.8 | 0.330 | 0.52 | 317 | 0.5 | 1.00 |
| 4 | 321 | 3.1 | 0.289 | 0.45 | 322 | 0.5 | 1.00 |
| 5 | 315 | 3.2 | 0.327 | 0.51 | 320 | 0.5 | 1.00 |
| 6 | 310 | 2.9 | 0.330 | 0.51 | 323 | 0.5 | 1.00 |
| 7 | 305 | 2.8 | 0.297 | 0.46 | 323 | 0.5 | 1.00 |
| 8 | 300 | 2.8 | 0.317 | 0.50 | 317 | 0.5 | 1.00 |
| 10 | 296 | 2.8 | 0.277 | 0.43 | 322 | 0.5 | 0.99 |
| 9 | 290 | 2.6 | 0.250 | 0.39 | 320 | 0.5 | 0.98 |
| 11 | 282 | 2.2 | 0.289 | 0.45 | 322 | 0.5 | 0.97 |
| 12 | 270 | 2 | 0.314 | 0.49 | 320 | 0.5 | 0.96 |
| 13 | 261 | 2.1 | 0.364 | 0.57 | 320 | 0.5 | 0.95 |
| Control | NA | NA | NA | NA | 319 | NA | NA |
NA, not applicable.
a
Center wavelength (±0.5 nm) and bandwidth about center were measured with spectrometer. Near-constant average dose is achieved by adjusting the laser power (±0.01 mW) after determining irradiation spot size (±0.005 cm2). In this way, irradiation time is kept almost constant.
b
Calculated dose based on irradiance in laser spot and exposure time.
c
Correction factor for average attenuation along 1-mm exposure path.