Figure 4.

Effect of long-term PACAP38 treatment on the expression of somatostatin and neprilysin in the brain of 6-mo-old APP[V717I]-transgenic mice. Animals were treated as described in Fig. 3. A, B) Quantification of somatostatin (A) and neprilysin (B) mRNA levels by quantitative real-time RT-PCR (n=7 animals/group). Quantification of mRNA levels was performed as described in Fig. 3. C) Representative Western blot and quantitative analysis of neprilysin expression; 80 μg of mouse brain membrane proteins was analyzed (n=12 animals/group). ***P < 0.001.