Figure 2.

B-vitamin status in blood and colonic tissue. Means represent pooled data from the BAT-lacZ and BAT-lacZ×Apc1638N models, since the values for the models do not differ significantly. Plasma and colonic folate concentrations were determined by a conventional microbiological microtiter plate assay using L. casei. Vitamin B₂ was measured by the erythrocyte glutathione reductase activity coefficient assay; B₁₂ was determined by a competitive protein binding assay. B₆ was not measured due to limited sample, although such data appears for the same diet in a prior publication (14). MVD, multiple vitamin depletion. Control, n = 19 mice; MVD, n = 21 mice.