Figure 1.

A) Schematic illustration depicting the chemical immobilization of aptamers onto the MSC surface using a 3-step procedure. B) Successful conjugation of aptamers on the MSC surface was confirmed by flow cytometry. A positive fluorescence signal was observed for MSCs modified with aptamer-dye, and the intensity of the signal is directly related to the concentration of aptamer used during the conjugation process. Control experiments using MSCs with aptamer-FAM that lacked biotin modification (FAM-L-aptamer; Table 1) exhibited minimal fluorescence (data not shown).