Figure 1.

ACE gene LD plots were analyzed separately for sprint and distance dogs and closely related breed pairs to determine if sled dogs have similar LD patterns to the domestic breeds. LD was determined for each group based on the 81 polymorphisms found from manual sequence screening of the 20 Alaskan sled dogs (10 sprint, 10 distance) and 80 purebred dogs. The LD plots were analyzed with the 4 gamete rule and depicted using the alternate D′/LOD color scheme in Haploview 4.1. Low LOD and high LOD (logarithm (base 10) of odds) scores with low D′ scores are in white, low LOD with high D′ scores are in shades of pink, and high LOD with high D′ scores are in black. (A) 10 sprint dogs; (B) 10 distance dogs; (C) 10 Alaskan Malamutes and 10 Siberian Huskies; (D) 10 Greyhounds and 10 Whippets (E) 10 Mastiffs and 10 Staffordshire Bull Terriers (F) 10 English Pointers and 10 German shorthaired Pointers. The Alaskan sled dogs demonstrate a substantial amount of LD in the ACE gene that differs between the sprint and distance populations. The purebred pairs show less LD in the ACE gene and no similarity in pattern to the Alaskan sled dog populations. (This figure appears in color in the online version of Journal of Heredity.)