FIG 5 .

DDX17 facilitates viral RNA synthesis in H5N1 infection of human cells. (A) 293T cells were targeted by Nontarget (Non) or DDX17 (DX17) siRNA. After 24 h of knockdown, cells were infected by A/VN/1203/04 (H5N1) HALo virus (MOI = 1). Cell protein lysates were analyzed 12 h p.i. for the DDX17, NP, M1, and actin proteins by Western blotting. (B) Primer extension analysis with specific primers for HA (i) or NP (ii) mRNA and vRNA synthesis, as indicated, for mock-infected control or 293T cells 12 h p.i. with A/VN/1203/04 (H5N1) HALo virus (MOI = 1). 5S, rRNA loading control; *, nonspecific background band. Graphs show RNA quantification by densitometry normalized to 5S rRNA and background; significance was estimated by an unpaired, 2-tailed t test from density histograms and standard deviations.