Figure 5. Correlation between PGE₂:PGD₂ ratio and respective enzyme transcript expression ratio.

(A) Eicosanoid ratios of PGE₂:PGD₂ after 8 h and 24 h KLA stimulation, where PGD₂, PGJ₂, 15d PGD₂, and 15d PGJ₂ were summed. (B) Transcript expression ratios of mPGES-1:H-PGDS using average expression of 0 h and 8 h time-points (0–8 h) and 0, 8, and 24 h time-points (0–24) in RPM (blue), TGEM (green), BMDM (purple), and RAW (red) macrophages stimulated with KLA (100 ng/ml). The data are expressed as mean values ± sem of three biological replicates.