Table 1. Total Macrophage Eicosanoid Production.
| Enzymatic pathway | Time poststimulation | KLA (–/+) | Eicosanoid production (pmol/μg DNA) |
|||
|---|---|---|---|---|---|---|
| Resident | TGEM | BMDM | RAW | |||
| COX | 8 h | – | 9.2 ± 1.6 | 5.4 ± 0.3 | 2.7 ± 0.4 | 2.3 ± 0.7 |
| + | 202.6 ± 12.2 | 31.4 ± 3.2 | 21.9 ± 4.7 | 432.4 ± 69.2 | ||
| 24 h | – | 14.8 ± 1.9 | 5.5 ± 0.4 | 1.9 ± 0.4 | 1.5 ± 0.4 | |
| + | 242.6 ± 45.0 | 40.3 ± 4.4 | 24.3 ± 5.7 | 438.0 ± 105.2 | ||
| 5-LOX | 8 h | – | 1.1 ± 0.3 | 0.0 | 0.0 | 0.0 |
| + | 2.5 ± 0.3 | 0.0 | 0.0 | 0.0 | ||
| 24 h | – | 2.2 ± 0.2 | 0.0 | 0.0 | 0.0 | |
| + | 6.4 ± 0.7 | 0.0 | 0.0 | 0.0 | ||
| 15-LOX | 8 h | – | 0.5 ± 0.0 | 0.2 ± 0.0 | 1.7 ± 0.1 | 0.6 ± 0.1 |
| + | 1.4 ± 0.2 | 0.3 ± 0.0 | 1.8 ± 0.3 | 0.7 ± 0.1 | ||
| 24 h | – | 1.0 ± 0.1 | 0.2 ± 0.0 | 0.9 ± 0.2 | 0.5 ± 0.2 | |
| + | 5.5 ± 0.6 | 0.3 ± 0.0 | 1.6 ± 0.4 | 0.2 ± 0.1 | ||
| 12-LOX | 8 h | – | 1.7 ± 0.2 | 1.0 ± 0.2 | 0.6 ± 0.1 | 1.1 ± 0.1 |
| + | 2.4 ± 0.1 | 1.3 ± 0.1 | 0.7 ± 0.2 | 2.1 ± 0.0 | ||
| 24 h | – | 4.8 ± 0.3 | 1.2 ± 0.1 | 0.6 ± 0.2 | 1.1 ± 0.3 | |
| + | 6.4 ± 0.5 | 2.1 ± 0.1 | 1.2 ± 0.4 | 1.5 ± 0.4 | ||
| CYP | 8 h | – | 1.3 ± 0.1 | 3.9 ± 0.8 | 1.4 ± 0.1 | 0.9 ± 0.1 |
| + | 1.4 ± 0.2 | 4.3 ± 0.5 | 1.2 ± 0.2 | 0.9 ± 0.1 | ||
| 24 h | – | 3.3 ± 0.3 | 9.6 ± 1.2 | 2.2 ± 0.3 | 1.2 ± 0.2 | |
| + | 2.3 ± 0.1 | 8.9 ± 1.3 | 1.5 ± 0.2 | 0.3 ± 0.1 | ||
Quantitative analysis of eicosanoids formed through COX, 5-LOX, 15-LOX, 12-LOX, and CYP pathways, expressed as pmol eicosanoid per μg DNA. The data are from the same experiment as in Fig. 1 and are expressed as mean values ± sem of three biological replicates.