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. 2011 Sep;90(3):591–597. doi: 10.1189/jlb.0910531

Figure 2. MCs in tissues distant from the nidus of infection degranulate after CLP.

Figure 2.

Percentage of degranulated MCs in selected tissues (A–D) determined by morphologic appearance (E, inset) of toluidine blue-stained MCs identified in sections of skin, trachea, stomach, and peritoneal lavage fluid in uninfected mice and then 4 h and 12 h or 24 h after CLP-induced infection (n=5–9 mice/group). (E) Photomicrograph of a representative section of skin 24 h after CLP stained with toluidine blue. Thick arrows indicate degranulated MCs, and thin arrows represent nondegranulated MCs, which show tightly packed cytoplasmic granules after toluidine blue staining (Non-degran), whereas degranulated MCs have less tightly packed granules, which can be seen spilling into the surrounding tissue (Degran). (F) Bacterial loads in the blood and peritoneum [peritoneal lavage (PL)] of mice, 4 h and 24 h after CLP. Error bars indicate sem; *P < 0.05.