| 10 bp ladder (1 µg/ µL) | 1 µL |
| 10× T4 PNK buffer | 1 µL |
| [γ-32P] ATP | 2 µL |
| T4 PNK | 1 µL |
| 0.1 M DTT | 0.4 µL |
| Water | 4.6 µL |
| Total volume | 10 µL |
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