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Representative images of two discordant cases that were considered automated quantitative analysis (AQUA) positive/immunohistochemistry (IHC) negative. (A) and (B) show case 1, and (C) and (D) show case 2. Both cases were visualized by immunofluorescence (A and C) or by conventional diaminobenzidine (DAB) stain with hematoxylin counterstain (B and D). CLIA, Clinical Laboratory Improvement Amendments; QIF, quantitative immunofluorescence.
