Fig. 3.

Extracellular regulated kinases (ERKs), Akt, and c-Jun N-terminal kinase (JNK) are persistently phosphorylated in human vestibular schwannoma (VS) cells. (A–C) Western blots of VS cell lysates from cultures maintained in serum-free, basal medium treated with the mitogen activated protein kinase kinase (MAPKK, MEK) MEK inhibitor U0126 (10 µM); the PI3-K inhibitor, LY294002 (20 µM); and/or the ErbB2 inhibitor PD158780 (100 nM) as indicated and probed with the indicated antibodies. (D) Immunostaining of cultured VS cells with anti-pJNK (red; upper and lower panel) and anti-S100 (green; lower panel). Scale bar = 50 µm.