Fig. 8.

siRNA-mediated c-Jun N-terminal kinase (JNK) 1/2 knock-down increases vestibular schwannoma (VS) cell apoptosis. VS cultures were transfected with a siRNA oligonucleotide targeting JNK1 and JNK2 (JNK1/2) or with a scrambled (Scr) oligonucleotide. (A) Western blot probed with the indicated antibodies demonstrating decreased JNK expression and Jun phosphorylation in cultures expressing JNK1/2-targeted siRNA oligonucleotide. (B) Apoptosis was scored as the percentage of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)–positive, S100-positive VS cells with condensed nuclei. The data are from cultures derived from 4 separate tumors. Error bars represent standard errors of the mean. P = .035, by the Student's 2-tailed t test.