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. Author manuscript; available in PMC: 2011 Aug 18.
Published in final edited form as: Proteomics Clin Appl. 2008 Sep 10;2(10-11):1518–1527. doi: 10.1002/prca.200800034

Figure 4.

Figure 4

Detection of autoantibodies by NAPPA ELISA is highly reproducible. Over 600 individual c-terminal GST tagged proteins were expressed in microarray format, Antigen expression across the slide was confirmed by anti-GST antibody (left). Slides containing anchored cDNAs were stored at room temperature, and proteins were expressed on consecutive days. Human IgG registration spots are visible as diagonal duplicates across the slide. P53 antigen was specifically detected in duplicate using human sera containing anti-p53 antibodies. The coefficients of variation for antibody detection were 7% (within day) and 11% (between days). The CV's were calculated based on the means of the replicate spots on each slide.