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. Author manuscript; available in PMC: 2011 Aug 18.
Published in final edited form as: Proteomics Clin Appl. 2008 Sep 10;2(10-11):1518–1527. doi: 10.1002/prca.200800034

Figure 5.

Figure 5

Proof of concept detection of antibodies in several disease states. A. Detection of immunodominant pathogen-derived antigens. A selected set of 264 genes predicted to be outer membrane proteins from the pseudomonas genome was expressed by NAPPA and probed with control serum or serum derived from an infected patient. A well documented response to the pseudomonal antigen, fliC, was specifically detected in the patient sera. B. The Francisella tularensis proteome was expressed on a NAPPA array. The array was probed with sera from a mouse infected with a live attenuated strain and from a control mouse. The immune responses were detected with an anti-mouse antibody. C. An array expressing 655 human genes in duplicate was probed with sera from a healthy control and from a patient with prostate cancer. Antibodies to the common viral antigen EBNA-1 are readily detected in both sera, but antibodies to Bcl2 are only detected in the prostate cancer patient serum. D. An array expressing 659 human genes was probed with sera from a diabetic patient and a nondiabetic control. Serum response to a well known antigen related to IDDM, GAD65, was observed only in the patient sera.