Figure 3.

Role of iROS generation in the induction of NF-κB and proinflammatory mediators. The activation of NF-κB measured by the NoShift II NF-κB assay and the induction of IL-6, IL-8, IL-1α, and ELAM-1 measured by real-time Q-PCR, were evaluated in pTM cells treated with H₂O₂ in the presence or absence of 5 μM Fccp. The data show percentage changes in cultures treated with Fccp and H₂O₂ compared to those treated with vehicle (ethanol) and H₂O₂. A double asterisk means p<0.01; three asterisks mean p<0.001 (n=3).