Table 1. Primer sequences used for real-time quantitative polymerase chain reaction analysis.
| Gene | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
| IL-1a |
NM_214029 |
AAGTGTTGACAGGCCGTATG |
TACCAGACTTCGCTCCCTCT |
| ELAM-1 |
NM_214268 |
CCCATGGAACACAACCTGTGCATT |
AGCTTTACACGTTGGCTTCTTGCC |
| IL-8 |
AB057440 |
AAACTGGCTGTTGCCTTCTT |
ATTTATGCACTGGCATCGGAA |
| IL-6 |
NM_214399 |
GCTTCCAATCTGGGTTCAAT |
CTAATCTGCACAGCCTCGAC |
| b-actin | AY550069 | AAGATCAAGATCATCGCGCCTCCA | TGGAATGCAACTAACAGTCCGCCT |
For each gene, the nucleotide sequences of the two specific primers were detailed. The gene accession number used to find both primers is indicated. Abbreviations are: IL-1α, interleukin-1 alpha; ELAM-1, vascular endothelial leukocyte-adhesion molecule-1; IL-8, interleukin-8; and IL-6, interleukin-6.