Skip to main content
. 2011 Aug 18;7(8):e1002231. doi: 10.1371/journal.pgen.1002231

Figure 7. Longitudinal extension of chromosome territories in mutants defective for SC assembly and chromosome dynamics mediated by PC–SUN-1 linkages.

Figure 7

(A) Three-dimensional rendered paint images of chromosome II in premeiotic and early pachytene nuclei from wild-type and chk-2(me64), him-3(gk149) and syp-1(me17) mutant germ lines. Three fluorophores are assigned as in Figure 1 (Alexa-488, 564 and 647 from the left (PC) end to the right (non-PC) end). In the panel showing wild-type pre-meiotic nuclei, DAPI counter stain is shown in white and a dotted line is used to encircle the domain of DAPI-stained chromatin within each nucleus; in other panels, DAPI is omitted to facilitate visualization of the paint signals. Note that chromosome II territories show obvious longitudinal extension in all of these strains. Scale is shown by the square grid in the background of each panel, with 6 µm as the length of each side of the unit square. (B) Numbers of painted segments per chromosome scored for chromosome II in nuclei from the pre-meiotic zone (Pm) and the early pachytene zone (Pa) of germ lines of the indicated genotype. For each genotype, nuclei from two gonads were scored; error bars indicate standard deviation.