Skip to main content
. 2011 Aug 18;6(8):e23239. doi: 10.1371/journal.pone.0023239

Figure 5. CD40 is required for CD40L-induced activation of NF-κB pathway in neutrophils.

Figure 5

(A) NF-κB nuclear translocation was assessed by immunofluorescence staining for NF-κB p65 (green) in wildtype and CD40-deficient neutrophils exposed to CD40L (1.0 µg/ml) for 1 h. Cell nuclei were detected by DAPI (blue). Scale bars: 50 µm. (B) NF-κB nuclear translocation was assessed and quantified as the percentage of the p65 nuclei-positively stained cells to the total cells. Similar results are obtained from three independent experiments. Data are expressed as mean ± SEM. ** P<0.01 versus WT.