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. 2011 Aug 18;6(8):e23314. doi: 10.1371/journal.pone.0023314

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Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A ClustalW2 alignment colored by Boxshade (ver. 3.21) between NlpE_Y.p (NCBI annotation YPK_1090) from Y. pseudotuberculosis YPIII and NlpE_E.c (b0192) from E. coli K-12 strain MG1655. (B) Sampling and detection of invasin and RovA at stationary phase in LB broth at 26°C followed the procedure detailed in the legend to Figure 7. Additionally, NlpE_E.c (derived from E. coli) or NlpE_Y.p (Y. pseudotuberculosis) over-production was controlled by the presence (+) or absence (−) of 0.2% (w/v) L-arabinose. The pBAD18 empty expression vector served as a control. The strains used were: parent with empty vector, YPIII/pIB102, pBAD18; parent with NlpE_E.c, YPIII/pIB102, pND18; parent with NlpE_Y.p, YPIII/pIB102, pJF027; cpxR null mutant with empty vector, YPIII08/pIB102, pBAD18; cpxR null mutant with NlpE_E.c, YPIII08/pIB102, pND18; cpxR null mutant with NlpE_Y.p, YPIII08/pIB102, pJF027. Molecular weights shown in parentheses are deduced from primary sequence.