Figure 1. Generation of twinfilin-2a knockout mice.

(A) Partial map of the wild-type Twf2 allele, the targeting construct and the recombinant allele, and locations of the probes. The white boxes show the exons of the twinfilin-2 gene and the shaded box the neomycin cassette, black box indicates the pWH9 vector. The expected fragment sizes of the wild type and the recombinant allele after digestion with EcoRI and subsequent hybridization with the indicated external probe are 8,6 and 3,5 kb, and with the indicated internal probe, 8,6 and 11 kb, respectively. (B) Southern blot analysis with the external probe of EcoRI digested mouse tail DNAs derived from progeny of heterozygous mating reveals the wild-type (8,6 kb) and the targeted Twf2 allele (3,5 kb). [+/+ wild type, +/− heterozygote, −/− knockout].