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. 2011 Aug 18;6(8):e22974. doi: 10.1371/journal.pone.0022974

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Relative expression of the budB transcript in potato stems and tubers was determined with four reference mRNA (ffh, dspE, pelB, and hrcC - Table 2). The mean relative expression ratio (RER) of the budB transcript in tubers was 8.5-fold elevated compared to stem tissue, which was used as the calibrator. The difference was found to be significant at a 95% confidence interval by t-test using Prism software (Mac version 5.0d, GraphPad Software, Inc.) with a P-value = 0.0425. Error bars represent standard error of the mean.