Table 2. RT-qPCR primer sequences and efficiency.
| Gene name | Primer sequence (5′→3′, forward to reverse) | Efficiency (%)A |
| budB | TTGAATCTGCTGATGAAC | |
| CAATGGTTATCGGAATAATC | 102.8 | |
| dspE | GTCCTATACCAACCTCAG | |
| GCAACGAAGAGAACAAAT | 98.8 | |
| ffh | TGGAAACATTGGCAGAGC | |
| GACTAACAAGACATCGTAGAAC | 105 | |
| hrcC | GCCTATTCTGCCGAACAA | |
| GCACCAAATCCACACCAT | 99.9 | |
| pelB | CTCCGTAACAACAACATT | |
| GTACTCTTCCAGTCATCT | 102.3 | |
| proC | GTGCGAATTATGCCAAAC | |
| TTATCTGCCTGACTGACG | 100.7 |
A
: Primer efficiencies were calculated from dilution curve with the MyiQ Cycler software.