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. 2011 Aug 18;6(8):e22974. doi: 10.1371/journal.pone.0022974

Table 4. Twenty-six treatments used to examine 3H2B (acetoin) and 23B (2, 3-butanediol) separately and in combination at various concentrations, in the presence or absence of lanolin, and at two application rates.

Treatment Compound Lanolin addition Compound conc. Rate applied
1 2,3-butanediol Yes 10 µg/ml 10 µl
2 2,3-butanediol Yes 100 ng/ml 10 µl
3 2,3-butanediol Yes 1 ng/ml 10 µl
4 2,3-butanediol Yes 10 pg/ml 10 µl
5 2,3-butanediol No 10 µg/ml 10 µl
6 2,3-butanediol No 100 ng/ml 10 µl
7 2,3-butanediol No 1 ng/ml 10 µl
8 2,3-butanediol No 10 pg/ml 10 µl
9 Acetoin Yes 1 mg/ml 10 µl
10 Acetoin Yes 10 µg/ml 10 µl
11 Acetoin Yes 100 ng/ml 10 µl
12 Acetoin Yes 1 ng/ml 10 µl
13 Acetoin No 1 mg/ml 10 µl
14 Acetoin No 10 µg/ml 10 µl
15 Acetoin No 100 ng/ml 10 µl
16 Acetoin No 1 ng/ml 10 µl
17 CombinationA No 10 µg/ml, 1 mg/ml 10 µl
18 Combination No 100 ng/ml, 10 µg/ml 10 µl
19 Combination No 1 ng/ml, 100 ng/ml 10 µl
20 Combination No 10 pg/ml, 1 ng/ml 10 µl
21 Combination No 10 µg/ml, 1 mg/ml 20 µl
22 Combination No 100 ng/ml, 10 µg/ml 20 µl
23 Combination No 1 ng/ml, 100 ng/ml 20 µl
24 Combination No 10 pg/ml, 1 ng/ml 20 µl
25 H20 No NA 10 µl
26 Solvent control No NA 10 µl
A

In combination treatments, the applied rate was added per compound with the 23B concentration listed first.