Table 4. Twenty-six treatments used to examine 3H2B (acetoin) and 23B (2, 3-butanediol) separately and in combination at various concentrations, in the presence or absence of lanolin, and at two application rates.
Treatment | Compound | Lanolin addition | Compound conc. | Rate applied |
1 | 2,3-butanediol | Yes | 10 µg/ml | 10 µl |
2 | 2,3-butanediol | Yes | 100 ng/ml | 10 µl |
3 | 2,3-butanediol | Yes | 1 ng/ml | 10 µl |
4 | 2,3-butanediol | Yes | 10 pg/ml | 10 µl |
5 | 2,3-butanediol | No | 10 µg/ml | 10 µl |
6 | 2,3-butanediol | No | 100 ng/ml | 10 µl |
7 | 2,3-butanediol | No | 1 ng/ml | 10 µl |
8 | 2,3-butanediol | No | 10 pg/ml | 10 µl |
9 | Acetoin | Yes | 1 mg/ml | 10 µl |
10 | Acetoin | Yes | 10 µg/ml | 10 µl |
11 | Acetoin | Yes | 100 ng/ml | 10 µl |
12 | Acetoin | Yes | 1 ng/ml | 10 µl |
13 | Acetoin | No | 1 mg/ml | 10 µl |
14 | Acetoin | No | 10 µg/ml | 10 µl |
15 | Acetoin | No | 100 ng/ml | 10 µl |
16 | Acetoin | No | 1 ng/ml | 10 µl |
17 | CombinationA | No | 10 µg/ml, 1 mg/ml | 10 µl |
18 | Combination | No | 100 ng/ml, 10 µg/ml | 10 µl |
19 | Combination | No | 1 ng/ml, 100 ng/ml | 10 µl |
20 | Combination | No | 10 pg/ml, 1 ng/ml | 10 µl |
21 | Combination | No | 10 µg/ml, 1 mg/ml | 20 µl |
22 | Combination | No | 100 ng/ml, 10 µg/ml | 20 µl |
23 | Combination | No | 1 ng/ml, 100 ng/ml | 20 µl |
24 | Combination | No | 10 pg/ml, 1 ng/ml | 20 µl |
25 | H20 | No | NA | 10 µl |
26 | Solvent control | No | NA | 10 µl |
In combination treatments, the applied rate was added per compound with the 23B concentration listed first.